Detergent Removal
Detergents may need to be removed before downstream LC or LC/MS analysis. The trapping approach depends on the detergent type and whether the detergent or the target protein or peptide should be retained during cleanup.
The SDS is retained by the trap while the protein or peptide passes through for downstream processing or analysis.
The protein or peptide is temporarily retained while the non-ionic detergent is flushed away, then the retained target is released.
For non-SDS ionic detergents, zwitterionic detergents, other detergent types, or an uncertain detergent identity, use the technical-help path below before selecting a chemistry.
Continue to OPTI-TRAP Cartridges For supported SDS and non-ionic detergent removal, continue to OPTI-TRAP Cartridges and complete the exact cartridge configuration on the product page. View OPTI-TRAP Cartridges →Small Molecule SDS Removal is not automatically equivalent to generic Small Molecule, SAX, or SCX chemistry. Non-Ionic Detergent Removal is not automatically equivalent to generic NID, SCX, SAX, or other similarly named phases. For detailed chemistry interpretation, use the Trap Chemistry Reference.
Detergent type, chemistry, target behavior, pressure, flow, connections, and method requirements can affect the appropriate setup. For non-ionic detergent removal, protein retention can also depend on the relationship between target pI and mobile-phase pH. Use technical assistance when these conditions make the appropriate chemistry or configuration unclear.
Use the Trap Compatibility & Holder Reference to confirm the correct cartridge and holder combination. If your primary objective is concentration or desalting rather than detergent removal, see Sample Concentration & Desalting.
Need help choosing the right approach? For non-SDS ionic, zwitterionic, other, or uncertain detergent types; unclear chemistry; pI/pH method-development questions; detergent-load or sizing uncertainty; system or method constraints; exact configuration questions; or an uncertain cartridge/holder pairing, contact Optimize Technologies.