Peptide Fractionation & 2D LC Trapping
This Optimize tandem trapping workflow uses two different trapping stages to fractionate peptides, transfer them to a second trap for concentration and desalting, and then elute them for downstream LC analysis.
SCX trapping/fractionation → controlled peptide release → peptide concentration/desalting → downstream elution and LC analysis
Used for the peptide fractionation stage before controlled release to the second trap.
Receives the released peptides for concentration and desalting before downstream elution and LC analysis.
These are application-specific chemistry identities for this workflow. Similarly named generic phases are not automatically equivalent. For detailed chemistry interpretation, use the Trap Chemistry Reference.
Continue to OPTI-TRAP Cartridges For this tandem workflow, use OPTI-TRAP Cartridges for the required trapping stages. Choose the required cartridge configurations on the product page. View OPTI-TRAP Cartridges →If pressure, flow, connections, method requirements, or another system constraint makes OPTI-TRAP unsuitable for your setup, contact Optimize Technologies before selecting another Trap family.
Use the Trap Compatibility & Holder Reference to confirm the correct cartridge and holder combination. If your need is standalone sample concentration or desalting rather than this tandem workflow, see Sample Concentration & Desalting.
Need help with this workflow? If your method differs from the workflow above, OPTI-TRAP does not meet your system requirements, the chemistry is unclear, or you need help determining the exact configuration or cartridge/holder pairing, contact Optimize Technologies.