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The EXP® Trap Column system is designed for on-line trapping in LC and LC/MS workflows, giving chromatographers a convenient way to clean up or concentrate samples before analysis.
The EXP Cartridge System can be used to remove detergents or salts that may interfere with ionization in MS work and to concentrate samples directly on-line. This approach can help preserve valuable sample material compared with off-line handling and readily supports automated or high-throughput UHPLC/MS trapping workflows.
EXP Trap Column cartridges are used with supported EXP holder configurations. With a Direct Connect holder, the assembled system connects directly to 10-32 injection-valve ports; with an In-Line holder, the system is plumbed with 1/16 in tubing. Free-Turn® holder architecture allows cartridges to be changed by hand without breaking the holder inlet/outlet fluid connections, while the holder's auto-adjusting connection provides a zero-dead-volume fluid path.
The EXP Trap Column system is rated to 20,000+ psi (1,400+ bar). Configuration-specific pressure limits apply; the 30.0 µm Protein configuration is limited to 6,000 psi. Operate within the pressure limit of the selected cartridge configuration.
Standard online ordering is available in 4 µL, 10 µL, 20 µL, 40 µL, and 100 µL bed volumes using the configurations shown in the product selectors.
Need a different size?
EXP Trap Column cartridges are available in 2 mm, 5 mm, 10 mm, 15 mm, 20 mm, and 30 mm lengths. The configurations shown in the product selectors are the standard 5 mm online-order options. For 2 mm, 10 mm, 15 mm, 20 mm, or 30 mm cartridges, or for custom packing and other nonstandard configurations, contact Optimize Technologies to discuss your application.
Current standard packing options include 3.0 µm EXP®, 2.7 µm HALO®, 5.0 µm HALO®, and a 30.0 µm Protein configuration with a 6,000 psi pressure limit. Available phases depend on the selected packing and cartridge configuration. The standard online selectors use 5 mm cartridges. For 2 mm, 10 mm, 15 mm, 20 mm, or 30 mm lengths, custom packing, or other nonstandard configurations, contact Optimize Technologies.
EXP Trap Column Cartridges are used with the following EXP holder configurations:
The EXP Trap Column Discovery Kit (15-02-04042) is a related purchase path that combines an EXP holder with EXP Trap Column cartridge packs.
Related product: EXP Guard Column Cartridges. EXP Trap and Guard cartridges can be used with the supported EXP holder configurations listed above.
Packing and phase availability depends on the selected configuration. Current standard phase options are listed under Technical Specs and in the product selectors.
For additional product-selection guidance:
For custom configurations or compatibility questions not covered by the available product information, contact Optimize Technologies before ordering.
| Bed Volume | Load Rate | Dimensions |
|---|---|---|
| 4 µL | 0.10-5 mL/min | 1.0 mm × 5 mm |
| 10 µL | 0.20-8 mL/min | 1.5 mm × 5 mm |
| 20 µL | 0.50-12 mL/min | 2.1 mm × 5 mm |
| 40 µL | 1-15 mL/min | 3.0 mm × 5 mm |
| 100 µL | 1-20 mL/min | 4.6 mm × 5 mm |
For estimating purposes, Optimize literature recommends approximately 4 µg of sample capacity per 1 µL of bed volume. Actual capacity depends on the application and conditions.
| Specification | Information |
|---|---|
| Standard pack quantity | 3 cartridges |
| Cartridge direction | Uni-directional |
| Available cartridge lengths | 2 mm, 5 mm, 10 mm, 15 mm, 20 mm, 30 mm |
| Current standard packing options | 3.0 µm EXP®, 2.7 µm HALO®, 5.0 µm HALO®, 30.0 µm Protein configuration |
| 3.0 µm EXP® phases | C18, C8, C4, HILIC, Phenyl-Hexyl, SAX, SCX |
| 2.7 µm HALO® phases | C18, C8, HILIC, RP-Amide, Phenyl-Hexyl, Peptide ES-C18 |
| 5.0 µm HALO® phases | C18, C8, HILIC, Phenyl-Hexyl, PFP, ES-CN, Penta-HILIC |
| System pressure | Rated to 20,000+ psi (1,400+ bar) |
| Pressure exception | 30.0 µm Protein configuration is limited to 6,000 psi. Operate within the pressure limit of the selected cartridge configuration. |
| Direct Connect configuration | EXP Direct Connect holder connects directly to 10-32 injection-valve ports |
| In-Line configuration | EXP In-Line holder is plumbed with 1/16 in tubing |
| Connection design | Auto-adjusting holder connection provides a zero-dead-volume fluid path |
| Cartridge changes | Free-Turn® holder design allows cartridges to be changed by hand without breaking inlet/outlet fluid connections |
Need a different size?
EXP Trap Column cartridges are available in 2 mm, 5 mm, 10 mm, 15 mm, 20 mm, and 30 mm lengths. Standard online ordering uses 5 mm cartridges. For 2 mm, 10 mm, 15 mm, 20 mm, or 30 mm cartridges, custom packing, or other nonstandard configurations, contact Optimize Technologies.
