Applications: Tricyclic Antidepressants

Here, five tricyclic antidepressants are separated isocratically on a 50mm Velocity column packed with Monitor C18.

Analytes

1. Protriptyline
2. Nortriptyline
3. Doxepin
4. Imipramine
5. Amitriptyline

Experimental Conditions

Column: Velocity 4.6x50mm
Packing: Monitor C18 5µm
Mobile Phase: 15:85 ACN:20mM Phosphate Buffer, pH 7.0
Flow Rate: 1mL/min
Detection: UV @ 254 nm