Applications: Caffeine, Sodium Benzoate and Aspartame in Diet Cola

A simple, effective assay for determination of three key ingredients found in many types of diet soda.

Analytes

1. Caffeine
2. Sodium Benzoate
3. Aspartame

Experimental Conditions

Column: Velocity 4.6x50mm
Packing: Monitor C18 5µm
Mobile Phase: 13:87 ACN:20mM Phosphate Buffer, pH 4.3, with 0.1% TEA
Flow Rate: 1mL/min
Detection: UV @ 254 nm