Applications: Antihistamines

Three common antihistamines are separated in a ph 2.5 Phosphate buffer with 20% acetonitrile on a 4.6 x 50 mm Velocity column.

Analytes

1. Pyrilamine
2. Chlorpheniramine
3. Brompheniramine

Experimental Conditions

Column: Velocity 4.6x50mm
Packing: Monitor C18 5µm
Mobile Phase: 20:80 ACN:20mM Phosphate Buffer, pH 2.5
Flow Rate: 1mL/min
Detection: UV @ 254 nm