Trap Columns

An Elegant and Efficient Solution For HPLC or LC/MS Analysis

Often a sample bound for HPLC or LC/MS analysis requires some type of clean-up to ensure optimal results. Detergents or salts can affect the ionization process, while overly dilute samples can be swamped out by background noise, making them undetectable. There are many methods to treat samples, but most require lengthy off-line procedures that involve significant sample handling. These procedures not only consume valuable time, but also can result in appreciable sample loss.

Online Desalting and Detergent Removal

Optimize Technologies trap columns are an elegant and efficient means for sample processing. Trap columns are small packed beds that use the adsorptive properties of analytes to effectively concentrate dilute samples, desalt, remove detergents, and more. Unlike off-line procedures that require significant sample handling and potential sample loss, Optimize trap columns have the advantage of being able to be used in a completely on-line environment. On-line clean up not only increases recovery, but is also amenable to automation for unattended use and higher throughput.

Traps work on the principle of "digital chromatography." This is the most apt description because there are typically only two states of the analyte: bound or unbound. This on/off behavior is exploited by either trapping the analyte and letting sample matrix and contaminants go to waste; or by trapping the undesirable components and allowing analytes to pass unimpeded. The discontinuous interaction of the analyte in a trap column prevents dilution and dispersion that may occur in larger beds with a more distributive partitioning effect.

Optimize Technologies offers two lines of trap columns to suit any trapping need. The OPTI-PAK® capillary traps are designed for ultra-low volume applications where any excess in volume could be detrimental to the assay. OPTI-LYNX™ traps offer higher capacity, as well as the OPTI-LYNX™ quick-connect hardware for the ultimate in connection simplicity. Both formats are available in a number of different phases and support materials; choose the trap design and material that best fits your needs.

Optimize Technologies also offers custom dimensions and packings. If you have a special need or application, give us a call. We are always ready and excited to apply our engineering, manufacturing, and scientific expertise to the solution of any problem.